READ ME FIRST!!!
MaChIAto (Microhomology-associated Chromosomal Integration/editing Analysis tools); a comprehensive analysis software that can precisely classify, deeply analyze, correctly align, and thoroughly review the targeted amplicon sequencing analysis data obtained by various CRISPR experiments, including template-free gene knock-out, short homology-based gene knock-in, and even a new-class CRISPR methodology, Prime Editing.
The source code is available at:
GitHub MaChIAto
Manuals
Quick Start
Setting
Usage
- Usage of MaChIAto Classifier
- Usage of MaChIAto Aligner
- Usage of collect_MaChIAto_data.py
- Usage of MaChIAto Analyzer
- Usage of MaChIAto Reviewer
Explanation of Output Files
- Output of MaChIAto Classifier
- Output of MaChIAto Aligner
- Output of collect_MaChIAto_data.py
- Output of MaChIAto Analyzer
- Output of MaChIAto Reviewer
Supplementary Code
References
1 Nakamae et. al, Detailed profiling with MaChIAto reveals various genomic and epigenomic features affecting the efficacy of knock-out, short homology-based knock-in and Prime Editing. bioRxiv. 2022 https://doi.org/10.1101/2022.06.27.496697
Contact Us
If you have a question and request, don’t hesitate to contact me: Kazuki Nakamae, Ph.D.(kazukinakamae@gmail.com).