MaChIAto (Microhomology-associated Chromosomal Integration/editing Analysis tools); a comprehensive analysis software that can precisely classify, deeply analyze, correctly align, and thoroughly review the targeted amplicon sequencing analysis data obtained by various CRISPR experiments, including template-free gene knock-out, short homology-based gene knock-in, and even a new-class CRISPR methodology, Prime Editing.
MaChIAto would be helpful for people who want to
- get a more accurate classification of knock-out, homology-based knock-in, and Prime Editing.
- quantify the rate of imprecise editing reads, including the reads with donor-reversed integration.
- obtain the local alignment of the target site and knock-in junction.
- analyze the sequence involved with InDels and imprecise editing.
- find the useful features for the efficiency prediction.